Hank's Balanced Salts is designed to equilibrate with air, hence does not require a CO2-air mixture environment. It does not contain calcium, magnesium, and phenol red.

All cell culture media have a basis of a synthetic mixture of inorganic salts known as the physiological or balanced salt solution (BSS). All the physiological salt solutions are derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt solution developed specifically for supporting the metabolism of mammalian cells was Tyrode's solution. Since then, many modifications have been done to obtain better buffering salt solutions and to prevent calcium precipitation.

Manufactured under ISO 13485 QMS and in compliance with applicable cGMP guidelines.