VitroGel® YIGSR High Concentration
Overview
VitroGel® YIGSR is a ready-to-use tunable hydrogel system modified with laminin-derived functional peptide (YIGSR). YIGSR sequence resides on the laminin β1 chain, which involves endothelial cell adhesion, cell proliferation and motility/migration.
Mix & Match – 3D Cell Culture Your WAY!
VitroGel® YIGSR is one of the families of the xeno-free tunable VitroGel system. This hydrogel can mix with other versions of VitroGel such as VitroGel® RGD, VitroGel® IKVAV, VitroGel® MMP and VitroGel® COL to create the customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists get full control to manipulate the micro-environment for 3D cell culture and more!
VitroGel closely mimics the natural extracellular matrix (ECM) environment, creating a functional and optimized environment to make cells feel like at home. The hydrogel system is ready-to-use at room temperature, has a neutral pH, transparent, permeable and compatible with different imaging systems. The hydrogel strength can be adjusted with VitroGel® Dilution Solution. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivostudies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.
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Cannot find the functional hydrogel you want? Contact us for a customized hydrogel system.
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Need rheology data of VitroGel with your medium and mixture for publication? Check our data support service.
Specifications
Contents | VitroGel® YIGSR High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL |
Hydrogel Formulation | Xeno-free tunable hydrogel modified with YIGSR peptide. HIGH CONCENTRATION |
Use | Support endothelial cell adhesion, cell proliferation and motility/migration |
Mix & Match | Can be blended with other versions of VitroGel to create a multi-functional hydrogel |
Operation | Room temperature |
Hydrogel Strength | 10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
pH | Neutral |
Color | Transparent |
Cell Harvesting | 20 min cell recovery using VitroGel Cell Recovery Solution |
Injectable | Injectable hydrogel |
Storage | Store at 2-8°C. Ships at ambient temperature |
Product Documentation
Data and References
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Figure 1. Rheological properties of VitroGel YIGSR with DMEM medium. A) The gel formation curve after mixing with DMEM medium. VitroGel YIGSR was diluted at 1:0,1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with DMEM at 4:1 (v/v) ratio; B) The gel strength after 24 hrs incubation. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa)
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Figure 2. 3D culture of glioblastoma cells (U-87 MG) in VitroGel YIGSR. Cells were cultured with 1:3 diluted VitroGel YIGSR according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).
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Figure 3. 3D culture of breast cancer cells (MCF-7) in VitroGel YIGSR. Cells were cultured with 1:3 diluted VitroGel YIGSR according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration)
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