VitroGel® MMP High Concentration
Overview
VitroGel® MMP is a ready-to-use tunable hydrogel system modified with matrix metalloproteinases sensitive peptide. The enzyme sensitive hydrogel can be degraded by several MMPs (MMP1, MMP2, MMP3, MMP9 and MMP13) and support different biological activities such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, etc.
Mix & Match – 3D Cell Culture Your WAY!
VitroGel® MMP is one of the families of the xeno-free tunable VitroGel system. This hydrogel can mix with other versions of VitroGel such as VitroGel® RGD, VitroGel® IKVAV, VitroGel® YIGSR, and VitroGel® COL to create the customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists get full control to manipulate the micro-environment for 3D cell culture and more!
VitroGel closely mimics the natural extracellular matrix (ECM) environment, creating a functional and optimized environment to make cells feel like at home. The hydrogel system is ready-to-use at room temperature, has a neutral pH, transparent, permeable and compatible with different imaging systems. The hydrogel strength can be adjusted with VitroGel® Dilution Solution. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivostudies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.
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Cannot find the functional hydrogel you want? Contact us for a customized hydrogel system.
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Specifications
Contents | VitroGel® MMP High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL |
Hydrogel Formulation | Xeno-free tunable hydrogel modified with matrix metalloproteinases sensitive peptide. HIGH CONCENTRATION |
Use | MMP sensitive biodegradable hydrogel. |
Mix & Match | Can be blended with other versions of VitroGel to create a multi-functional hydrogel |
Operation | Room temperature |
Hydrogel Strength | 10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
pH | Neutral |
Color | Transparent |
Cell Harvesting | 20 min cell recovery using VitroGel Cell Recovery Solution |
Injectable | Injectable hydrogel |
Storage | Store at 2-8°C. Ships at ambient temperature |
Product Documentation
Data and References
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Figure 1. Rheological properties of VitroGel MMP with RPMI medium. A) The gel formation curve after mixing with RPMI medium. VitroGel MMP was diluted at 1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with RPMI at 4:1 (v/v) ratio; B) The gel strength after 24 hrs incubation. The hydrogel was prepared according to the user handbook at 1:0, 1:1 and 1:3 dilution ratios, and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G' of regular products at dilutions. Customized high concentration product to reach over 20K Pa)
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Figure 2. Degradation of VitroGel MMP gel with MMP2 enzyme. A) The hydrogel was prepared without dilution (1:0 dilution) and incubated for 24 hrs before adding MMP2 (1 µg/mL) on the top of the hydrogel. The plate was incubated in a 5% CO2 incubator at 37°C and was shaken using an orbital shaker at 40 rpm. The hydrogel appearance starts getting chunky after 24 hrs and then by Day 6, the entire middle section has degraded; B) The 1:3 diluted hydrogel was prepared with VitroGel Dilution Solution and incubated as 1:0 diluted hydrogel. The hydrogel appearance shows high degradation after 6 hrs after exposed to MMP2 enzyme. C). The rheological data shows a rapid decline in the elastic modulus of the 1:3 diluted hydrogel overtime after adding MMP2 enzyme. The gel was almost completely degraded by the enzyme after 200 mins. The test result demonstrates that VitroGel MMP undergoes an MMP-sensitive degradation. The higher the dilution of the gel, the faster the degradation process.
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Figure 3. 3D culture of glioblastoma cells (SNB 75) in VitroGel MMP. Cells were cultured with 1:3 diluted VitroGel IKVAV according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).
Mix & Match Hydrogel System – 3D Cell Culture Your Way
Mix VitroGel RGD with VitroGel MMP
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Figure 4. 3D culture of mouse bone marrow stromal cells (OP9, mesenchyme) in the mixture of VitroGel RGD and VitroGel MMP. The mixing ratio of VitroGel RGD and VitroGel MMP is 3:1 (V/V). Cells were cultured with 1:3 diluted VitroGel mixture according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).
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